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Marek's disease virus in Egypt: historical overview and current research based on the major mdv-encoded oncogene meq

An update survey of the MDV strains circulating in the Egyptian poultry flocks during 2015 -2016 was conducted. Twenty one vaccinated chicken flocks suffering from different form of lymphomas, representing different governorates in the Egyptian Delta, were screened for MDV infection. Pathological findings in the visceral tumours and presence of viral DNAs with variable mitotic figures were observed in the examined visceral organs. Eight samples were screened positive for the major MDV-encoded oncogene Meq which was subjected for alignment and phylogenetic analysis.

An ex vivo chicken primary bursal-cell culture model to study infectious bursal disease virus pathogenesis

Infectious bursal disease virus (IBDV) is a birnavirus of economic importance to the poultry industry. The virus infects B cells, causing morbidity, mortality, and immunosuppression in infected birds. In this study, we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with IBDV, and the quantification of viral replication. The addition of chicken CD40 ligand significantly increased cell proliferation fourfold over six days of culture and significantly enhanced cell viability.

Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs

Co-infection with avian leukosis virus subgroup J and reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown.

Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein

Newcastle disease virus (NDV) V protein is crucial for viral interferon (IFN) antagonism and virulence, determining its host range restriction. However, little information is available on the B cell epitopes of V protein and the subcellular movement of V protein in the process of NDV infection. In this study, the monoclonal antibody (mAb) clone 3D7 against genotype VII NDV V protein was generated by immunizing mice with a purified recombinant His-tagged carboxyl-terminal domain (CTD) region of V protein.

Spotlight on avian pathology: Marek’s disease

Marek's disease (MD), characterised by rapid-onset lymphoid tumours, immunosuppression and paralysis, is one of the most widespread economically important disease of poultry world-wide. Caused by the highly contagious Marek's disease virus (MDV), control of MD is mainly achieved through vaccination with live attenuated vaccines, although improvements in genetic resistance have also been an important component of disease control.

Broccoli meal fed to laying hens increases nutrients in eggs and deepens the yolk color

The nutritious stems and leaves of broccoli often go to landfills as byproducts after harvesting and processing of the florets. The stems and leaves contain specific carotenoids that are noted to have anti-allergic, anti-cancer and anti-obesity bioactivity. Research has shown the stems and leaves could be made into a meal, small amounts of which could be added to poultry diets to increase the nutrients in eggs and also deepen the color of the yolks. We studied adding a relatively high percentage (15%) of broccoli stem and leaves meal to the corn-soy diets of White Leghorn inbred crosses.

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Global distributions and strain diversity of avian infectious bronchitis virus: a review

The poultry industry faces challenge amidst global food security crisis. Infectious bronchitis is one of the most important viral infections that cause huge economic loss to the poultry industry worldwide. The causative agent, infectious bronchitis virus (IBV) is an RNA virus with great ability for mutation and recombination; thus, capable of generating new virus strains that are difficult to control. There are many IBV strains found worldwide, including the Massachusetts, 4/91, D274, and QX-like strains that can be grouped under the classic or variant serotypes.

Marek's disease virus infection of phagocytes: a de novo in vitro infection model

Marek’s disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful.

Early pathogenesis during infectious bursal disease in susceptible chickens is associated with changes in B cell genomic methylation and loss of genome integrity

We propose a model by which an increase in the genomic modification, 5-hydroxymethylcytosine (5hmC), contributes to B cell death within the chicken bursa of Fabricus (BF) infected with infectious bursal disease virus (IBDV). Our findings indicate that, following an IBDV infection, Rhode Island Red (RIR) chickens have fewer surviving B cells and higher levels of 5hmC in the BF than the more resistant 15l line of birds. Elevated genomic 5hmC levels within the RIR BF are associated with markers of immune responses: infiltrating T cells and increased expression of CD40L, FasL and iNOS.

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